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The expression of the CD45 (PTPRC) gene product is controlled by three alternative promoters (pA, pB, pC), which lead to generation of isoforms via alternative exon usage. On the basis of similar protein sequences, we assumed that mouse cells would express all three possible isoforms of CD45 whereas human cells would mainly express CD45RA and CD45RO isoforms. The expression of CD45 isoforms was analyzed in human peripheral blood T cells and in murine CD8 cells. All CD8 T cells derived from mice expressed only two CD45 isoforms, CD45RB and CD45RA, with the predominance of the CD45RB isoform. The vast majority of human peripheral blood CD8 T cells expressed CD45RB isoform and to a lesser extent also CD45RA isoform. CD45 isoform-specific antibodies were used to analyze the lymphocytic subsets of human and mouse peripheral blood. The analysis revealed that the majority of human CD8 cells co-expressed CD45RB and CD45RA isoforms, whereas in mouse, the majority of CD8 cells did not express any CD45 isoform. Although the expression of CD45 isoforms in mouse CD8 cells is similar to that in human T cells, the expression of CD45 isoforms in peripheral blood lymphocytes seems to be a universal characteristic of mice.Candidates, donors in OK race are not required to release tax returns, according to IRS guidelines

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